ultrasonic homogenizer

The 500 W ultrasonic homogenizer safely handle a wide range of organic and inorganic materials, from 250 microliters to 1 liter*.

Typical applications include nanotechnology (production of nanoparticulate materials and graphene dispersions), cell lysis, sample preparation, homogenization, ChIP analysis, emulsification, depolymerization and depolymerization, and applications in the field of sonochemical processing.

● Energy and temperature set point control

● Digital circuit + 7 inch wide screen (touch)

● Automatic frequency modulation accurate to 1Hz + real-time monitoring of sample temperature

● 99 hours working time setting

● Variable power output control can be adjusted arbitrarily from 1 to 99%

● Electric lift + jog setting

● Frequency automatic search accuracy ±1Hz and real-time display

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PDF:Cell Disruption

Methods of cell disruption:

1. Mechanical crushing method: refers to the use of a masher, a grinder or a homogenizer to break the cells apart.

1. High-speed tissue mashing: make the material into a thin paste liquid, place it in about 1/3 of the volume of the cylinder, close the cylinder lid tightly, turn the governor to the slowest position first, turn on the switch, and gradually accelerate to the desired position. speed required. This method is suitable for animal visceral tissue, plant succulent seeds, etc.

2. Homogenization by glass homogenizer: first place the chopped tissue in a tube, then insert the grinding rod into the tube to grind it back and forth, and move it up and down to grind the cells. High, suitable for small amount and animal organ tissue.

2. Physical fragmentation method: refers to the use of temperature difference, pressure difference or ultrasonic wave to break cells apart.

1: Treat the cell suspension with a certain power of ultrasonic wave, so that the cells are violently shaken and broken (the cell wall and organelles are broken by the vibration force of the ultrasonic wave).

Mechanism: It may be related to the cavitation phenomenon of bubble generation, growth and fragmentation when strong sonic waves act on the solution, and the shock wave and scissor force caused by the cavitation phenomenon cause cell lysis.

The efficiency of sonication depends on the sound frequency, sound energy, treatment time, cell concentration and cell type, etc. (Pay attention to cooling down when using to prevent overheating).

2. High-pressure fragmentation: The cell suspension is ejected from the annular gap of the high-pressure chamber onto the stationary impact ring, and is forced to change direction and flow out through the outlet tube. During this process, the cells undergo high-speed shear collisions and changes from high pressure to atmospheric pressure, thereby breaking up and releasing the contents.

This is an ideal method for gentle, thorough cell disruption.

3. Repeated freeze-thaw method: freeze the cells below -20 degrees, thaw at room temperature, and repeat several times. Due to the formation of ice particles in the cells and the increase of the salt concentration of the remaining cell fluid, the swelling will be caused, and the cell structure will be broken.

3. Chemical fragmentation method: refers to the use of formaldehyde, acetone and other organic solvents or surfactants to act on the cell membrane, so that the structure of the cell membrane is damaged or the permeability is changed.

Some animal cells, such as tumor cells, can be destroyed by the use of sodium dodecyl sulfonate (SDS), sodium deoxycholate and other cell membranes. The concentration is generally 1 mg/ml.

4. Enzymatic fragmentation method: Use appropriate enzymes to destroy the cell wall, and then fragment the protoplasts in a hypotonic solution.

Bacterial cell walls are thicker and can be treated with lysozyme for better results.

Standard formulation of lysate: : 50mM Tris-HCl (pH8.5~9.0), 2mM EDTA, 100mM NaCl, 0.5% Triton X-100, 1mg/ml lysozyme. (Lysozyme works better in this pH range).

Comprehensive description: No matter which method is used to disrupt tissue cells, intracellular proteins or nucleic acid hydrolase will be released into the solution, which will biodegrade macromolecules, resulting in a reduction in the amount of natural substances. Adding diisopropyl fluorophosphoric acid (DFP) Can inhibit or slow down autolysis; the addition of iodoacetic acid can inhibit the activity of those proteolytic enzymes that require a sulfhydryl group in the active center, and the addition of phenylmethanesulfonyl fluoride (PMSF) can also eliminate the activity of proteolytic enzymes, but not all, And it should be added several times while crushing; in addition, pH, temperature or ionic strength can also be selected to make these conditions suitable for the extraction of the target substance.

Precautions before use:

1. Select an appropriate probe according to the volume of the sample to be processed. Before and after use, the probe must be wiped with an alcohol cotton ball, and the probe must be replaced with a special wrench;

2. When the AMPLITUDE knob is turned on, the probe must not be exposed to the air, and in special cases (test probe) should not exceed 10 seconds;

3. Prepare an ice box before use, and the sample must be placed in an ice bath during processing, and the sample concentration should not be too large.

The 500 W ultrasonic homogenizer safely handle a wide range of organic and inorganic materials, from 250 microliters to 1 liter*.

Typical applications include nanotechnology (production of nanoparticulate materials and graphene dispersions), cell lysis, sample preparation, homogenization, ChIP analysis, emulsification, depolymerization and depolymerization, and applications in the field of sonochemical processing.